Load a plasmid

Click here or drag and drop a DNA file (.gb / .gbk / .ape / .fasta / .fastq) anywhere on this page.

Plasmid

Feature Types

Selection:
(overrides per-feature custom colors while checked)
Type Label Location Strand Size
No enzymes enabled

Matches

Enzyme Site Temp Strand Site location Top-strand cut Bottom-strand cut

Predicted gel

Enable one or more enzymes above to see predicted fragments and a virtual gel. Ladder: NEB 1 kb Plus DNA Ladder.

Alignment viewer

Drop .gb / .gbk / .fasta / .ab1 files here to align against the current reference. Pre-computed consensus assemblies (Plasmidsaurus, pLannotate, etc.) work best; Sanger .ab1 reads are treated as partial reads.
Drop reads here
No reads loaded. Click + Add reads or drop files above.

Primer annealing

Primers collected from every plasmid in your vault, matched against this plasmid's sequence (both strands, wraps at origin if circular). Exact full-length binding only.

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